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. 2020 Dec 14;21(24):9506. doi: 10.3390/ijms21249506

Figure 1.

Figure 1

miR166 knockdown mediates its target genes up-regulated expression and leaf rolling in maize. (A) The diagram of STTM166 structure with a 48 nt length spacer and two non-cleavable miRNA binding sites. (B) Morphology of C01 and STTM166 seedlings. Bar = 10 cm. (C) qRT-PCR analysis of miR166 expression levels in C01 and STTM166 plants. (D) Analysis of miR166 target genes’ expression levels. U6 small nuclear RNA and Actin were used as the internal control of miR166 and target genes in qRT-PCR analysis, respectively. ** represents that the corresponding miR166 and target gene expression levels of STTM166 plants are very significantly different from the wild type p < 0.01, respectively. Bars show standard error of phenotypic values.