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. 2020 Dec 14;12(12):2983. doi: 10.3390/polym12122983

Table 1.

Fumonisin B1 (FB1), aflatoxin B1 (AFB1) and ochratoxin A (OTA) aptamers and relative buffer composition.

Target Mycotoxin Aptamer Name ssDNA Sequence (5′-3′) Buffer Composition Reference
FB1 FB1_39 ATACCAGCTTATTCAATTAATCGCATTACCTTATACCAGCTTATTCAATTACGTCTGCACATACCAGCTTATTCAATTAGATAGTAAGTGCAATCT 100 mM NaCl, 20 mM Tris, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6 [25]
FB1_39t3 ATACCAGCTTATTCAATTAATCGCATTACCTTATACCAGCTTATTCAATTACGTCTGCACATACCAGCTTATTCAATT Same as FB1_39 [26]
FB1_10 AGCAGCACAGAGGTCAGATGCGATCTGGATATTATTTTTGATACCCCTTTGGGGAGACATCCTATGCGTGCTACCGTGAA 100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02 % Tween 20 (pH 7.4) [27]
AFB1 AF_AB3 ATCCGTCACACCTGCTCTATTCCTCTGTTGAAGAACCACTTCCGGAAATAAGAGTGGTGTTGGCTCCCGTAT 100 mM NaCl, 20 mM Tris–HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2 [20]
AF_APT1 AGCAGCACAGAGGTCAGATGGTGCTATCATGCGCTCAATGGGAGACTTTAGCTGCCCCCACCTATGCGTGCTACCGTGAA 100 mM NaCl, 20 mM Tris–HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20 (pH 7) [19]
OTA 1.12.2 GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA 10 mM HEPES, 120 mM NaCl, 5 mM KCl, 10mM CaCl2, pH 7.0 [14]
A08 AGCCTCGTCTGTTCTCCCGGCAGTGTGGGCGAATCTATGCGTACCGTTCGATATCGTGGGGAAGACAAGCAGACGT 10 mM Na2HPO4, 2 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, pH 7.4 [15]
A08min GGCAGTGTGGGCGAATCTAT
GCGTACCGTTCGATATCGTG
Same as A08 [15]
H8 GGGAGGACGAAGCGGAACTGGGTGTGGGGTGATCAAGGGAGTAGACTACAGAAGACACGCCCGACA 10 mM Na2HPO4, 2 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, pH 7.4 [16]
H12 GGGAGGACGAAGCGGAACCGGGTGTGGGTGCCTTGATCCAGGGAGTCTCAGAAGACACGCCCGACA Same as H8 [16]