. 2020 Dec 14;12(12):2983. doi: 10.3390/polym12122983

Table 3.

Dissociation constant (K_D) values and corresponding free energy (ΔG⁰) values calculated by using Fluorescent Microscale Thermophoresis (MST) and magnetic beads (MBs) assays of selected aptamer towards target mycotoxins. The standard error values of the calculated K_D values were derived from two independent experiments.

Target Mycotoxin	Aptamer Name	MST Assay		MBs Assay
Target Mycotoxin	Aptamer Name	K_D (nmol/L)	ΔG⁰ Energy ^a (kcal/mol)	K_D (nmol/L)	ΔG⁰ Energy (kcal/mol)
Fumonisin B1 (FB1)	FB1_39	31 ± 22	−10.24 ± 0.42	1.53 ± 0.67	−12.03 ± 0.26
	FB1_39t3	2200 ± 1100	−7.72 ± 0.30	NB ^b	- ^c
	FB1_10	162 ± 137	−9.26 ± 0.50	NB	-
Aflatoxin B1 (AFB1)	AF_AB3	178 ± 50	−9.21 ± 0.17	NB	-
Aflatoxin B1 (AFB1)	AF_APT1	NB	-	1.40 ± 0.51	−12.08 ± 0.22
Ochratoxin A (OTA)	1.12.2	525 ± 147 ^d	−8.57 ± 0.17	374 ± 255 ^e	−8.77 ± 0.40
	A08	233 ± 81 ^d	−9.05 ± 0.21	286 ± 149 ^e	−8.93 ± 0.31
	A08min	97 ± 33 ^d	−9.57 ± 0.20	406 ± 166 ^e	−8.72 ± 0.24
	H8	416 ± 119 ^d	−8.70 ± 0.17	14 ± 7 ^e	−10.71 ± 0.30
	H12	630 ± 80 ^d	−8.46 ± 0.08	40 ± 14 ^e	−10.09 ± 0.21

^a Free energy values calculated according to the formula: ${∆ G}^{0} = - R T l n (K_{D});$ ^b NB = no binding detected; ^c not applicable; ^d McKeague et al. [39]; ^e McKeague et al. [31].