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. 2020 Dec 14;9(12):1271. doi: 10.3390/antiox9121271

Figure 4.

Figure 4

Effect of garcinol on cytokine production, immune cell accumulation, acetylation of nuclear factor-κB (NF-κB) p65 in cisplatin-injected mice. (A) Plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). (B) The mRNA expression of TNF-α and IL-6. (C) Immunofluorescent staining of kidney tissues for Ly6B.2. Scale bar = 50 μm. (D) Number of Ly6B.2-stained cells. (E) Immunohistochemical staining of kidney tissues for galectin-3. Scale bar = 50 μm. (F) Number of galectin 3-stained cells. (G) The mRNA expression of chemokine (C-X-C motif) ligand 1 (CXCL1), monocyte chemoattractant protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). (H) Immunoblotting of ICAM-1. (I) Quantification of immunoblot for ICAM-1. (J) Immunoblotting of acetyl-NF-κB p65 (Lys310). (K) Quantification of immunoblot for acetyl-NF-κB p65. n = 8 per group. *** p < 0.001 vs. Veh. ## p < 0.01 and ### p < 0.001 vs. CP.