Figure 4.

Coffee infusions protect by Yap1-GFP nuclear localization during oxidative stress inducing by hydrogen peroxide. Control—cells from the Yeast Extract–Peptone–Dextrose (YPD) culture and 1 mM H₂O₂—cells incubated for 1 h with hydrogen peroxide in the YPD medium. Yap1-GFP fusion protein can be seen to concentrate in the nuclei and coffee + H₂O₂—cells incubated for 3 h with coffee, centrifuged and hanging in fresh medium, and treated with hydrogen peroxide for 1 h. Representative results from three independent experiments are shown. Fluorescence pictures were taken with an Olympus BX-51 microscope equipped with a DP-72 digital camera and cellSens Dimension software (1000× magnification).