Table 3.
Sensitivity of the BY4741 strain and isogenic mutant strains sod1Δ, sod2Δ and rad52Δ treated with Arabica or Robusta coffee infusions on cells stressed factors.
| Strain | Growth Conditions | |||||
|---|---|---|---|---|---|---|
| Congo Red 15 μg/mL | Congo Red 15 µg/mL + Arabica | Congo Red 15 µg/mL + Robusta | Calcofluor White 20 µg/mL | Calcofluor White 20 µg/mL + Arabica | Calcofluor White 20 µg/mL + Robusta | |
| BY4741 | ++++ | ++++ | +++ | ++++ | ++++ | ++++ |
| sod1Δ | ++ | +++ | ++++ | ++ | ++++ | ++++ |
| sod2Δ | ++++ | ++++ | +++ | ++++ | ++++ | ++++ |
| rad52Δ | +++ | ++++ | ++++ | +++ | +++ | ++++ |
| Strain | NaCl 0.5 M | NaCl 0.5 M + Arabica | NaCl 0.5 M + Robusta | CH3COOH 40 mM | CH3COOH 40 mM + Arabica | CH3COOH 40 mM + Robusta |
| BY4741 | ++++ | +++ | ++ | +++ | +++ | ++ |
| sod1Δ | ++++ | +++ | + | ++ | ++ | + |
| sod2Δ | ++++ | +++ | +++ | +++ | ++++ | ++ |
| rad52Δ | ++++ | +++ | ++ | +++ | ++++ | +++ |
| Strain | MMS 0.03% | MMS 0.03% + Arabica | MMS 0.03% + Robusta | Control | ||
| BY4741 | +++ | ++++ | ++++ | ++++ | ||
| sod1Δ | ++ | ++++ | +++ | ++++ | ||
| sod2Δ | ++++ | ++++ | ++++ | ++++ | ||
| rad52Δ | - | - | - | ++++ | ||
Cells were cultured overnight in liquid YPD medium, counted and diluted serially to obtain suspensions at the densities 107, 106, 105 and 104 cells/mL. Five microliters of each suspension was spotted at YPD solid medium containing Arabica or Robusta coffee infusions and analyzed toxic factors. Plates were cultivated 2 days in 28 °C. Each ‘‘+’’ means the growth of one spot containing respectively: 50,000, 5000, 500 and 50 cells, "-" means no growth.