Table 1.
Callus induction percentage of P. bullata midrib explants cultured on Murashige and Skoog (MS) basal medium treated with different types of auxins at different concentrations under light and dark conditions.
| Plant Growth Regulators (PGRs) | Light Condition | Dark Condition | ||||
| Callus Induction Percentage (%) | Induction Time (Days) | Callus Morphology | Callus Induction Percentage (%) | Induction Time (Days) | Callus Morphology | |
| 0 | - | - | - | - | - | - |
| 9.05 µM 2,4-D | - | - | - | - | - | - |
| 18.10 µM 2,4-D | - | - | - | 2.22 d | 130 | Compact |
| 27.14 µM 2,4-D | 2.22 d | 111 | Compact | - | - | - |
| 36.19 µM 2,4-D | - | - | - | - | - | - |
| 45.24 µM 2,4-D | - | - | - | 2.22 d | 94 | Compact |
| 8.28 µM picloram | 11.11 cd | 82 | Friable | - | - | - |
| 16.56 µM picloram | - | - | - | 31.11 a | 66 | Friable |
| 24.85 µM picloram | - | - | - | 13.33 cd | 66 | Friable |
| 33.13 µM picloram | 2.22 d | 67 | Friable | 28.89 bc | 66 | Friable |
| 41.41 µM picloram | - | - | - | - | - | - |
| 10.74 µM NAA | - | - | - | 17.78 bcd | 103 | Friable |
| 21.48 µM NAA | - | - | - | 11.11 cd | 101 | Friable |
| 32.22 µM NAA | - | - | - | 15.56 bcd | 98 | Friable |
| 42.96 µM NAA | - | - | - | 4.34 d | 96 | Friable |
| 53.70 µM NAA | 2.22 d | 90 | Friable | - | - | - |
| 9.05 µM dicamba | - | - | - | - | - | - |
| 18.10 µM dicamba | - | - | - | - | - | - |
| 27.14 µM dicamba | - | - | - | - | - | - |
| 36.19 µM dicamba | - | - | - | - | - | - |
| 45.24 µM dicamba | - | - | - | - | - | - |
Different letters indicate the values of significant difference at p ≤ 0.05 Tukey’s range test for light and dark conditions. (-) = no response.