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SM switches expression of EMT-associated molecular markers to epithelial ones in TGF-β stimulated A549 cells. The cells were treated with TGF-β (50 ng/mL) and/or SM (0.5 µM) for 48 h followed by evaluation of E-cadherin, vimentin and fibronectin mRNA (A) and ZO-1 protein (B) expression using RT-PCR and flow cytometry, respectively. Relative expression levels in A were shown as mean ± standard deviation from two independent experiments performed in tetraplicate.
