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. 2020 Dec 15;12(12):1213. doi: 10.3390/pharmaceutics12121213

Table 3.

HPLC conditions for each molecule.

Drug Mobile Phase Run Time
(min)
Retention Time (min) Wavelength
(nm)
Aspirin (A) methanol 30%, (B) 0.1% acetic acid 70% 20 8.5 210
Galantamine (A) acetonitrile 25%, (B) 0.1% TFA 75% 10 2.4 220
Sel-HCl (A) Methanol 35%, (B) 0.1% acetic acid 65% 10 2.2 210
Insulin (A) Acetonitrile 70%, (B) 0.1% TFA 30% 15 2.2 210
Caffeine (A) methanol 55%, (B) 0.1% acetic acid 45% 5 2.4 274
HC (A) methanol 70%, (B) 0.1% acetic acid 30% 6 2.5 254
HC-HS (A) Methanol 70%, (B) 0.1% acetic acid 30% 10 4.5 254