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. 2020 Dec 15;21(24):9523. doi: 10.3390/ijms21249523

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Purification of SARS-CoV-2 3CLpro. Figure shows a Coomassie-stained gel image, after SDS-PAGE analysis of samples: (1) Molecular weight standard; (2) Soluble lysate; (3) Ni-NTA affinity chromatography, flowthrough; (4) Ni-NTA affinity chromatography, eluate fraction of His₆-SARS-CoV-2 3CLpro; (5) ion-exchange affinity chromatography, eluate of untagged SARS-CoV-2 3CLpro. Dotted arrow shows SARS-CoV-2 3CLpro, which has a slightly lower molecular weight than the His₆-tagged enzyme.