Figure 5.
Cleavage of recombinant proteins by SARS-CoV-2 3CLpro. After cleavage reactions by SARS-CoV-2 PR, reaction mixtures were analyzed by SDS-PAGE. Uncleaved proteins and SARS-CoV-2 PR are shown by continuous and dashed lines, respectively, while asterisks indicate cleavage products. (a) Cleavage of His6-MBP-TSAVLQ*SGFRKM-mEYFP. After in-gel renaturation, the full-length substrate and SGFRKM-mEYFP cleavage product were visualized UV illumination, and the gel was stained with Coomassie dye, as well. (b) Cleavage reaction of recombinant BSA. (c) Cleavage reaction of recombinant plasminogen. (d) Cleavage of reaction recombinant IRAK1. (e) Cleavage reaction of recombinant PTK6. (f) Cleavage reaction of recombinant CTBP1. Gel images are representatives of ≥2 parallel experiments.