Figure 9.
Determination of cleavage sites by MALDI-TOF MS. In cleavage reactions His6-MBP-TSAVLQ*SGFRKM-mEYFP (a), recombinant CTBP1 (b), and His6-MBP-REGTRVQ*SVEQIRE-mEYFP proteins (c) were used as substrates. Molecular weights (Da) of SARS-CoV-2 3CLpro are labeled by black, values for the full-length substrates are shown by dark blue color, while the proteolytic fragments are colored by light blue and green, respectively. Cleavage site sequences are also shown, asterisks indicate cleavage position.