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(a) Serum osmolality; (b) serum sodium concentration; and (c) serum potassium concentration. First bout of 75 min cycle: E0 and E75; 5 h recovery: R0–R300; end of pre-load: P45; completion of 20 km time trial: end. Values are the mean ± SE. * SD significantly different from WA (p < 0.05). # DD significantly different from WA (p < 0.05).
