Effect of 7-octenoic acid, oleamide, and 1-phenyl-2-pentanol in vitro cell migration. (A) MDA-MB-231 cells were scratch wounded and incubated with compounds, doxorubicin, or complete medium (control). The wound areas were imaged at 0, 6, 12, and 24 h post-scratching. (B) Wound area (%) summarized from triplicate data. One-way ANOVA test was performed with multiple comparison corrections (Dunnett test). Data represent the mean ± SEM of three independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001). (C) Apoptosis and (D) cell cycle analysis by Muse cell analyzer. Cells were treatment with compounds for 24 h in all experiments. 7-Oct, 7-octenoic acid; 1-Phe, 1-Phenyl-2-pentanol; Ole, oleamide; dox, doxorubicin.