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. 2020 Dec 15;13(12):464. doi: 10.3390/ph13120464

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bioassay-guided fractionation and identification of potential anticancer compounds from MOL extract against TNBC cell lines MDA-MB-231. Crude EtOAc extract was fractionated (no.1–11) and screened for anticancer activities with different in vitro bioassay tests. The strongest fraction was selected for sub-fractionation and identification of bioactive compounds using LC-ESI-QTOF-MS/MS. Three candidate compounds (1-phenyl-2-pentanol, oleamide, and 7-octenoic acid) exhibited anticancer effects through inducing cell cycle arrest. Additionally, suppression of Bcl-2 resulting in the disruption of mitochondrial membrane permeability and subsequent release of apoptogenic factors such as cytochrome c, which activates caspase 3 and finally causes cell apoptosis (** p < 0.01, *** p < 0.001).