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. 2020 Dec 15;21(24):9527. doi: 10.3390/ijms21249527

Figure 6.

Figure 6

Comparative qRT-PCR analysis of LOX genes in Spirodela polyrhiza 7498, Lemna minor 8627, and Landoltia punctata 5562. Plants were taken for expression analysis at different ages of culture. All cultures were grown for 28 days in nutrient solution and samples were collected at 14, 21, and 28 days of growth. The SpACT and Sp18SrRNA housekeeping genes were used to normalize the expression of LOX genes, as described in Materials and Methods. (A) LOX1, (B) LOX3, (C) LOX4, (D) LOX6, and (E) LOX7 expression comparison was made among the three strains. ANOVA with Dunnett’s multiple comparisons test was performed for significant differences in LOX gene expression in the aging tissues among the duckweed species. Statistical significance between aging expression data points was assessed against the 14-day expression profiles and categorized as ** p < 0.01, using Graph Pad (version 8.0); ns: not significant.