Figure 8.

Comparative qRT-PCR analysis of LOX genes in Spirodela in response to exogenous salt. Five fronds of S. polyrhiza 7498 were grown for 14 days; then 200 mM NaCl solution was added; and samples were harvested at 0, 1, 3, 6, and 12 h [36]. Gene expression data were analyzed by qRT-PCR. The experiment was repeated three times (n = 3) with each sample size of approximately 100 fronds. Analysis of variance (ANOVA) with Dunnett’s multiple comparisons test was performed for significant differences. Statistical significance between data points was assessed against 0 h versus other time points of expression profiles using graph pad (version 8.0).