Table 3.
Factors and levels of Box–Behnken design.
| Independent Variables | Levels | ||
|---|---|---|---|
| −1 | 0 | 1 | |
| Enzymolysis temperature (°C) (X1) | 50.00 | 55.00 | 60.00 |
| pH (X2) | 4.50 | 5.00 | 5.50 |
| Enzymolysis time (min) (X3) | 90.00 | 120.00 | 150.00 |
| Enzyme dose (mL) (X4) | 15.00 | 20.00 | 25.00 |
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