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. 2020 Dec 15;8(12):2001. doi: 10.3390/microorganisms8122001

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell-associated as well as cell-free viremia (Cq-values) of sheep experimentally infected with either (A,B) SPPV-“India/2013/Surankote” or (C,D) SPPV-“Egypt/2018”. EDTA blood and serum samples were examined using the pan-capripox real-time qPCR. Thereby, (A,C) cell-associated and (B,D) cell-free viremia was analyzed. Animals were inoculated via three different routes: intravenous infection (black), intranasal infection (red) and transmission between infected and naïve animals (blue). Cut-off was set at Cq 40.0. # displays animals that were euthanized before the end of the study due to ethical reasons.