. 2020 Dec 15;10(12):1675. doi: 10.3390/biom10121675

Table 2.

Changes in the activity of nitrogen metabolizing enzymes glutamate dehydrogenase (GDH), glutamine synthetase (GS), nitrate reductase (NR), and glutamate synthase (GOGAT) expressed as units/mg protein in the shoots and roots of soybean, kidney bean, chickpea, lentil, and pea under control (cont.) and after enrichment with biologically active actinomycete isolates. Values are the average of three individual replicates (means ± S.D). Different letters represent significant differences between the treatments in each crop (Duncan test; p < 0.05; n = 4).

	GDH		GS		NR		GOGAT
Plant-Isolate	Root	Shoot	Root	Shoot	Root	Shoot	Root	Shoot
Soybean-Cont	0.97 ± 0.09 ^a	0.47 ± 0.03 ^a	1.99 ± 0.49 ^a	0.95 ± 0.21 ^a	0.84 ± 0.01 ^a	0.22 ± 0.01 ^a	1.82 ± 0.06 ^a	0.59 ± 0.05 ^a
Soybean-I2	0.91 ± 0.08 ^a	0.49 ± 0.08 ^a	6.56 ± 0.39 ^b	1.45 ± 0.04 ^a	1.53 ± 0.15 ^a	0.20 ± 0.02 ^a	2.39 ± 0.04 ^b	0.63 ± 0.05 ^a
Soybean-I8	0.93 ± 0.09 ^a	0.80 ± 0.11 ^a	6.70 ± 0.39 ^b	2.32 ± 0.08 ^b	1.09 ± 0.18 ^a	0.27 ± 0.01 ^a	1.53 ± 0.02 ^b	0.64 ± 0.05 ^a
Soybean-I12	1.20 ± 0.11 ^a	0.69 ± 0.08 ^a	8.31 ± 0.49 ^b	1.63 ± 0.09 ^a	1.56 ± 0.02 ^b	0.32 ± 0.02 ^b	3.22 ± 0.10 ^b	0.75 ± 0.06 ^a
Soybean-I15	0.97 ± 0.09 ^a	0.97 ± 0.18 ^a	6.99 ± 0.41 ^b	1.17 ± 0.11 ^a	1.87 ± 0.08 ^b	0.28 ± 0.01 ^a	3.80 ± 0.25 ^b	0.67 ± 0.05 ^a
Kidney Bean-Cont	0.92 ± 0.05 ^a	0.51 ± 0.00 ^a	2.63 ± 0.21 ^a	1.67 ± 0.24 ^a	0.05 ± 0.01 ^a	0.13 ± 0.02 ^a	1.59 ± 0.23 ^a	6.91 ± 0.65 ^a
Kidney Bean-I2	1.07 ± 0.08 ^a	0.91 ± 0.13 ^a	7.56 ± 0.45 ^b	1.69 ± 0.11 ^a	0.15 ± 0.00 ^b	0.20 ± 0.02 ^a	2.54 ± 0.04 ^a	9.83 ± 0.76 ^a
Kidney Bean-I8	1.10 ± 0.08 ^a	1.43 ± 0.23 ^a	7.72 ± 0.45 ^b	2.81 ± 0.24 ^a	0.09 ± 0.01 ^a	0.31 ± 0.02 ^b	1.62 ± 0.08 ^a	10.05 ± 0.78 ^a
Kidney Bean-I12	1.36 ± 0.10 ^a	1.00 ± 0.12 ^a	9.66 ± 0.57 ^b	2.50 ± 0.10 ^a	0.17 ± 0.00 ^b	0.41 ± 0.02 ^b	3.38 ± 0.06 ^b	10.05 ± 0.78 ^a
Kidney Bean-I15	1.14 ± 0.09 ^a	0.80 ± 0.02 ^b	8.05 ± 0.47 ^b	1.73 ± 0.06 ^a	0.20 ± 0.00 ^b	0.32 ± 0.02 ^b	3.38 ± 0.05 ^b	10.48 ± 0.81 ^a
Chickpea-Cont	1.07 ± 0.08 ^a	0.49 ± 0.01 ^a	2.37 ± 0.49 ^a	1.33 ± 0.13 ^a	0.15 ± 0.05 ^a	0.15 ± 0.01 ^a	0.85 ± 0.15 ^a	0.76 ± 0.06 ^a
Chickpea-I2	0.90 ± 0.07 ^a	0.73 ± 0.17 ^a	6.03 ± 0.36 ^b	1.15 ± 0.04 ^a	0.52 ± 0.01 ^b	0.13 ± 0.01 ^a	1.83 ± 0.03 ^b	0.93 ± 0.07 ^a
Chickpea-I8	0.92 ± 0.07 ^a	1.03 ± 0.12 ^a	6.16 ± 0.36 ^b	1.67 ± 0.05 ^a	0.36 ± 0.02 ^a	0.14 ± 0.01 ^a	1.27 ± 0.04 ^a	0.95 ± 0.07 ^a
Chickpea-I12	1.29 ± 0.10 ^a	0.80 ± 0.10 ^a	7.90 ± 0.46 ^b	1.95 ± 0.08 ^a	0.49 ± 0.01 ^b	0.18 ± 0.01 ^a	2.00 ± 0.03 ^b	1.00 ± 0.08 ^a
Chickpea-I15	0.96 ± 0.07 ^a	0.79 ± 0.09 ^a	6.42 ± 0.38 ^b	0.97 ± 0.03 ^a	0.70 ± 0.01 ^b	0.14 ± 0.01 ^a	2.48 ± 0.04 ^b	0.99 ± 0.08 ^a
Lentil-Cont	0.88 ± 0.05 ^a	0.47 ± 0.02 ^a	2.07 ± 0.29 ^a	1.31 ± 0.07 ^a	0.39 ± 0.07 ^a	0.16 ± 0.01 ^a	0.41 ± 0.01 ^a	1.43 ± 0.36 ^a
Lentil-I2	1.05 ± 0.09 ^a	0.91 ± 0.07 ^b	5.08 ± 0.30 ^b	1.06 ± 0.08 ^a	1.43 ± 0.18 ^b	0.19 ± 0.04 ^a	0.60 ± 0.03 ^b	1.23 ± 0.31 ^a
Lentil-I8	1.07 ± 0.09 ^a	0.96 ± 0.18 ^a	5.19 ± 0.31 ^b	1.42 ± 0.15 ^a	0.72 ± 0.08 ^a	0.22 ± 0.03 ^a	0.50 ± 0.04 ^a	3.25 ± 0.23 ^a
Lentil-I12	1.31 ± 0.11 ^a	0.58 ± 0.07 ^a	6.28 ± 0.37 ^b	1.22 ± 0.07 ^a	1.73 ± 0.20 ^b	0.28 ± 0.04 ^a	0.73 ± 0.02 ^b	1.74 ± 0.44 ^a
Lentil-I15	1.12 ± 0.09 ^a	0.80 ± 0.05 ^b	5.41 ± 0.32 ^b	0.93 ± 0.04 ^a	1.34 ± 0.21 ^a	0.23 ± 0.03 ^a	0.78 ± 0.01 ^b	1.43 ± 0.31 ^a
Pea-Cont	0.62 ± 0.09 ^a	0.55 ± 0.02 ^a	2.71 ± 0.44 ^a	1.03 ± 0.09 ^a	0.30 ± 0.07 ^a	0.12 ± 0.01 ^a	1.21 ± 0.11^a	3.87 ± 0.39 ^a
Pea-I2	1.27 ± 0.08 ^b	0.89 ± 0.07 ^b	6.37 ± 0.38 ^b	1.22 ± 0.09 ^a	1.33 ± 0.21 ^a	0.20 ± 0.03 ^a	4.81 ± 0.22 ^b	6.70 ± 0.52 ^a
Pea-I8	1.30 ± 0.09 ^b	0.72 ± 0.03 ^a	6.51 ± 0.38 ^b	1.66 ± 0.17 ^a	0.98 ± 0.19 ^a	0.27 ± 0.01 ^b	4.02 ± 0.32 ^b	6.84 ± 0.53 ^a
Pea-I12	1.47 ± 0.10 ^b	0.60 ± 0.07 ^a	6.76 ± 0.40 ^b	1.25 ± 0.07 ^a	1.98 ± 0.01 ^b	0.29 ± 0.01 ^b	5.70 ± 0.18 ^b	9.55 ± 0.74 ^b
Pea-I15	1.36 ± 0.09 ^b	0.86 ± 0.04 ^b	6.79 ± 0.40 ^b	1.11 ± 0.04 ^a	1.82 ± 0.35 ^a	0.28 ± 0.01 ^b	5.91 ± 0.10 ^b	7.13 ± 0.55 ^b