Table 2.
Differentially expressed miRNAs between PCOS patients and oocyte donor samples selected for RT-qPCR validation.
| A) MGC PCOS vs. donors: | ||
| miRNA | Log2 Fold Change | miRNA role in ovary |
| hsa-miR-205-5p | 4.82 | Expression is upregulated in MI oocytes upon IGF-1 treatment [22]. Upregulated in ovarian cancer (OC) cells compared to control group and is associated with poor survival rates. Proposed miRNA targets are SMAD4 and PTEN [23]. |
| hsa-miR-203a-3p | 3.56 | Expression levels are higher in granulosa cells of young women with normal ovarian reserve compared to young women with diminished ovarian reserve [24]. |
| hsa-miR-196a-5p | 2.49 | Detectable in bovine granulosa cells at day 3 but not at day 7 of the estrous cycle [25]. |
| hsa-let-7c-5p | 1.45 | Expressed higher in human CGC compared to MGC cells. miRNA expression is decreased in granulosa cells of early and progressive atretic follicles and in case of premature ovarian failure syndrome (measured from plasma) [26]. |
| hsa-miR-181a-5p | −1.56 | Expressed higher in human CGC compared to MGC [27]. In mouse granulosa cells miR-181a-5p targets ACVR2A (Activin Receptor IIA) and inhibits granulosa cell proliferation [28]. In oxidative stress conditions miRNA expression is upregulated in mouse granulosa cells and mediates granulosa cell apoptosis [29]. |
| B) FF PCOS vs. donors: | ||
| miRNA | Log2 Fold Change | miRNA role in ovary |
| hsa-miR-509-3-5p | 2.80 | Expression is higher in FF of PCOS patients compared to controls [30]. |
| hsa-miR-200c-3p | 2.26 | Expression is higher in granulosa cells [31] as well as in FF samples [32] of PCOS patients compared to control group. |
| hsa-miR-223-3p | 2.02 | EVs obtained from FF show expression of hsa-miR-223-3p [33]. miRNA expression is decreased in cumulus cells of PCOS patients [34]. |
| hsa-miR-1908-5p | −1.48 | Low expression predicts poor prognosis for ovarian cancer [35]. |
| hsa-miR-146a-5p | −1.81 | Expression is higher in human MGC samples compared to CGC [36]. |
| C) EV PCOS vs. donors: | ||
| miRNA | Log2 Fold Change | miRNA role in ovary |
| hsa-miR-200c-3p | 2.77 | Expression is higher in granulosa cells samples [31] as well as in FF [32] obtained from PCOS patients compared to control group. |
| hsa-miR-100-5p | 1.42 | Associated with cell proliferation regulation [37]. Downregulated in young women with diminished ovarian reserve compared to normal ovarian reserve [37]. |
| hsa-miR-17-5p | −1.55 | Expression is downregulated in granulosa cells and FF of PCOS women compared to controls [38]. miRNA expression is detected in EVs obtained from FF [33]. |