Figure 2.

(A) Uptake of radiolabelled conjugates normalized to uptake of [⁶⁸Ga]Ga-TAFC of each experiment, respectively. Grey bars represent control with [⁶⁸Ga]Ga-TAFC. Uptake can be blocked with [Fe]TAFC due to competition for uptake by the MirB transporter. Iron-repleted conditions [Fe (+)] lead to a decrease in MirB biosynthesis and active uptake into the hyphae. (B) Competition assay of [⁶⁸Ga]Ga-TAFC blocked with iron-containing antifungal conjugates in iron-depleted fungal culture. For all compounds a reduction of [⁶⁸Ga]Ga-TAFC uptake could be observed, indicating a specific interaction with the MirB transporter.