Glycemic control, group and gender effects on TGFβ1 serum levels. The M-ANOVA post-hoc analysis shed light on fixed factors (independent variables) effects on all analyzed dependent variables. A multivariate-ANOVA guided the stratification of TGFβ1 levels in terms of glycemic control and gender. (A) Both males and females, with reported poor glycemic control at enrollment, showed significantly (p < 0.05) higher levels of TGF1β, compared to other patients. The stratification of TGFβ1 on the basis of gender and subject group (B) showed that only PDR females treated with aflibercept had significantly (p < 0.05) higher levels of TGFβ1, compared to males of the same group. * p < 0.05 vs. “good glycemic control” group; # p < 0.05 males vs. females.