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. 2020 Dec 15;10(12):1678. doi: 10.3390/biom10121678

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental and analytical workflow. (left): Clinical PD samples (human omental biopsies) of pediatric PD patients and healthy controls; (right): in vitro samples from three different primary HUVEC cultures exposed to diluted conventional PD fluid (PDF) containing or lacking 8 mM alanyl-glutamine (AlaGln), or to control cell culture medium. TMT: tandem mass tags; ESI: electrospray ionization; MALDI: matrix-assisted laser desorption/ionization; IPS: internal pooled standard; 2D RP/RP LC-MS: two-dimensional reversed phase/reversed phase liquid chromatography tandem mass spectrometry; 2D-DiGE: two-dimension difference gel electrophoresis.