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. 2020 Dec 16;21(24):9575. doi: 10.3390/ijms21249575

Figure 5.

Figure 5

Upregulation of Akt-related transcription factors in natural killer (NK) cells by resveratrol. (a) NK92 cells were deprived of interleukin (IL)-2 for 24 h and then treated with IL-2 (5 ng/mL) with or without 20 μM resveratrol for 30 min. Data are shown as the mean ± SD of duplicate measurements. (b) The mRNA levels of c-Myb in NK92 cells after resveratrol treatment for 24 h measured using real-time polymerase chain reaction and calculated using the ΔΔCT method. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) was used as an internal control. (c) Western blot showing c-Myb expression. Data are shown as the mean ± SEM of three independent experiments. (d) Western blot showing c-Myb expression after treatment with the Akt inhibitor (MK-2206) and mammalian target of rapamycin (mTOR) inhibitor (KU-0063794). Data are shown as the mean ± SEM of three independent experiments. Asterisks indicate statistical significance using one-way ANOVA: * p < 0.05, *** p < 0.001, ns: not significant (p > 0.05).