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. 2020 Dec 16;10(12):207. doi: 10.3390/bios10120207

Table 2.

Absolute values for energy required to denature a double stranded helix. The energy required for probe to target is shown on the yellow diagonal (100% match between probe and target). Those figures shown in red represent potential cross-reaction and those in red and boxed, the non-target signal is close to or greater than the target and are cross-reactions. Probes for the clades of Prorocentrum lima are to the right of the vertical bar.

1 5 6 8 9 11 12 17 38 39 40 41 42 43 44 45 46
AO GA GS GE CM CCD1 CCD2 GnOst PLMo1 PLMo2 PlMo3 PlMo4 PlMo5 Mor1C1 Mor1C2 Mor1C3 Mo2C2
AO −475.7 −60.5 −59.4 −61.6 −36.7 −54.9 −50.7 −48.6 −42.3 −31.3 −48 −50.6 −40.4 −47.3 −57.1 −44.3 −31.3
GA −63.5 −470.2 −65.9 −39.5 −55.5 −60.8 −57.3 −52.2 −34.6 −40.2 −46.7 −64.4 −57.7 −63.6 −119.1 −56.5 −40.2
GS −52.2 −48.7 −484.1 −98.6 −38 −49.1 −48.6 −63.3 −50.8 −37.1 −53.2 −51.9 −51.5 −54.6 −73.8 −50.1 −37.1
GE −69.1 −52.9 −58.1 −472 −29.5 −35.7 −45.3 −69.4 −59.1 −31.3 −54.1 −56.2 −33.2 −41.4 −51.4 −50.7 −31.3
CM −35.8 −53.3 −34.6 −50.7 −422.6 −38.7 −35.2 −87.1 −60.4 −87.9 −38.3 −44.1 −31 −30.6 −64.4 −70.2 −87.9
Ccf.Can −38.7 −39.6 −30.7 −51.6 −39.1 −303.8 −77.3 −40.4 −60.8 −33 −46.7 −49.2 −83.3 −52.2 −52.8 −43.6 −33
CCan −50.1 −57.8 −48.6 −44.2 −42 −48.7 −524.6 −55.1 −44.1 −34.3 −46.4 −44.1 −58.3 −74.6 −53.5 −80.8 −34.3
GnOst −49.4 −69.8 −42.4 −77.3 −36.9 −58.3 −53.9 −328.3 −46.5 −60 −130.6 −61.6 −33.1 −59.3 −55.2 −46.2 −60
PLMo1 −60.2 −79.9 −36 −66.7 −65.4 −82 −39.8 −51.9 −389.1 −36.1 −58.9 −269.8 −80.4 −40.1 −115.2 −47.2 −36.1
PLMo2 −43 −35.3 −91.5 −36.7 −77.6 −41.3 −31.5 −111.6 −44.7 −392.9 −149.9 −49.9 −70 −64.4 −53.6 −51.9 −381.2
PlMo3 −47.2 −48.8 −49.8 −45.1 −41.5 −65.1 −49 −79.3 −84.2 −118.2 −467.8 −61.3 −45.3 −56.2 −55.4 −138.3 −114.9
PlMo4 −53.3 −36.8 −55.9 −39.9 −46.4 −51.5 −42.5 −71.1 −281.6 −65 −58.9 −384.1 −54.8 −57.4 −61.3 −44.8 −65
PlMo5 −45.8 −40.3 −26.8 −74.6 −27 −85.5 −90.3 −53 −44.2 −69 −58.6 −44.2 −463.2 −291 −41.4 −79.1 −69
Mor1C1 −45.8 −56.5 −51.3 −47 −37 −137.1 −114.9 −53 −50.9 −50.5 −115.8 −69.1 −283.5 −452 −41.4 −54.7 −50.5
Mor1C2 −49.1 −94.4 −66.1 −62.3 −69.7 −30.3 −55.6 −93.2 −136.4 −82.3 −48 −51.3 −46.4 −46.4 −391.1 −41.7 −82.3
Mor1C3 −24.5 −45.2 −44.8 −69.5 −38.5 −67.5 −77.3 −40 −51.3 −34.3 −125.9 −38.9 −27.7 −27.7 −44.9 −416.3 −34.3
Mo2C2 −43 −35.3 −91.5 −36.7 −77.6 −41.3 −31.5 −111.6 −60.1 −382.5 −139.5 −49.9 −70 −64.4 −53.6 −28.2 −378.6