Figure 2.

FKBPL expression is increased concomitantly with CD44 downregulation and VCAM-1 and ICAM-1 upregulation in preeclampsia. Immunofluorescence staining of placentae from women with and without preeclampsia (n = 4) was performed using an anti-FKBPL rabbit anti-human polyclonal primary antibody (A) at a 1:150 dilution and an anti-CD31 guinea pig anti-human polyclonal primary antibody (B) at 1:50. Four images per slide were taken at 20× magnification and the mean fluorescence quantified using Image J (2-tailed unpaired t-test, n = 4). RNA was extracted from the frozen placental tissue, converted to complementary deoxyribonucleic acid before FKBPL and CD44 (C) or VCAM-1 and ICAM-1 (D) mRNA expression was measured using qPCR. The mRNA expression was adjusted to 18S and to healthy controls (n = 3; 2-way ANOVA with Sidak’s multiple comparison test). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001. Abbreviation: PE, preeclampsia.