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Purification of peptide fractions by reversed-phase high-performance liquid chromatography (RP-HPLC) analysis of 5–30% ACN digested soy fraction. (a) The analysis was performed with a linear gradient of 0–40% ACN over 20 min (12 mL/min flow rate). Fractions were collected on the basis of their absorbance at λ = 280 nm. (b) Percentage of ACN elution of each fraction.
