Figure 2.

Cell viability and antioxidant activity of peptide fractions in Caco-2 cells. Cells were treated with peptide fractions (0.05 mg/mL) for 24 h. (a) The 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) test was carried out in the absence (blue bars) and in the presence (red bars) of 250 µM TbOOH (see Materials and Methods) to evaluate cell viability, and values re expressed as percentage of cell viability with respect to control. (b) CM-H₂DCFDA was utilized for monitoring ROS production and the reported fluorescence was measured at 90 min (a.u.) in the absence (blue bars) or in presence (red bars) of 200 µM TbOOH. Values are indicated as mean ± SD of eight replicates of three independent experiments. *** p < 0.001, ** p < 0.01, * p < 0.05.