Table 2.
Molecular docking analysis of soy peptides.
| Sequence | Peptide | MW (Da) | Class of Amino Acids (%) | pI (Theoretical) |
||||
|---|---|---|---|---|---|---|---|---|
| Basic | Acid | Neutral | Hydrophobic | |||||
| DEQIPSHPPR | D-10-R | 1174.56 | 20 | 20 | 50 | 10 | 5.32 | |
| NALEPDHRVESEGG | N-14-G | 1508.67 | 14.29 | 28.57 | 35.71 | 21.43 | 4.4 | |
| SLVNNDDRDS | S-10-S | 1133.48 | 10 | 30 | 40 | 20 | 3.93 | |
| KEQQQEQQQEEQPL | K-14-L | 1768.81 | 7.14 | 28.57 | 57.14 | 7.14 | 4.09 | |
| FVDAQPQQKEEG | F-12-G | 1374.63 | 8.33 | 25 | 41.67 | 25 | 4.14 | |
| VNPESQQGSPR | V-11-R | 1197.56 | 9.09 | 9.09 | 72.73 | 9.09 | 5.97 | |
| IGINAENNQRN | I-11-N | 1241.60 | 9.09 | 9.09 | 54.55 | 27.27 | 6 | |
| FGREEGQQQGEE | F-12-E | 1392.58 | 8.33 | 33.33 | 50 | 8.33 | 4.09 | |
| HEQKEEHEWHRKEE | H-14-E | 1929.86 | 42.86 | 42.86 | 7.14 | 7.14 | 5.39 | |
| MRKPQQEEDDDDE | M-13-E | 1633.64 | 15.38 | 53.85 | 23.08 | 7.69 | 3.9 | |
| GKHQQEEENEGGSI | G-14-I | 1540.66 | 14.29 | 28.57 | 50 | 7.14 | 4.48 | |
| YLAGNQEQE | Y-9-E | 1050.45 | 0 | 22.22 | 44.44 | 33.33 | 3.79 | |
| YQEPQESQQRG | Y-11-G | 1348.59 | 9.09 | 18.18 | 63.64 | 9.09 | 4.53 | |
| RQNIGQNSSPD | R-11-D | 1214.55 | 9.09 | 9.09 | 72.73 | 9.09 | 5.84 | |
| NNQLDQTPR | N-9-R | 1084.51 | 11.11 | 11.11 | 66.67 | 11.11 | 5.84 | |
| KQGQHQQEEEEEGGSV | K-16-V | 1797.76 | 12.5 | 31.25 | 50 | 6.25 | 4.32 | |
| VGLKEQQQEQ | V-10-Q | 1185.58 | 10 | 20 | 50 | 20 | 4.53 | |
| VGLKEQQQEQQ | V-11-Q | 1313.64 | 9.09 | 18.18 | 54.55 | 18.18 | 4.53 | |
| QRIPAGTT | Q-8-T | 842.45 | 12.5 | 0 | 62.5 | 25 | 9.75 | |
| LKVREDENNP | L-10-P | 1212.59 | 20 | 30 | 30 | 20 | 4.68 | |
| NKVDENGTPKPS | N-12-S | 1284.62 | 16.67 | 16.67 | 58.33 | 8.33 | 6.07 | |
| FNENESGDQV | F-10-V | 1137.44 | 0 | 30 | 50 | 20 | 3.57 | |
| RNLQGENEEEDSGA | R-14-A | 1546.63 | 7.14 | 35.71 | 42.86 | 14.29 | 3.91 | |
| IKPPTDEQQQRPQE | I-14-E | 1692.83 | 14.29 | 21.43 | 57.14 | 7.14 | 4.68 | |
| FSREEGQQQGEQ | F-12-Q | 1421.60 | 8.33 | 25 | 58.33 | 8.33 | 4.25 | |
| WQEQQDEDEDE | W-11-E | 1449.50 | 0 | 63.64 | 27.27 | 9.09 | 3.26 | |
| TLVNNDDRDS | T-10-S | 1147.49 | 10 | 30 | 40 | 20 | 3.93 | |
| QREEQEWPRKEEK | Q-13-K | 1770.85 | 30.77 | 38.46 | 23.08 | 7.69 | 5.07 | |
Soy peptides were evaluated with molecular docking analysis for their interaction in silico with Keap1. A score was assigned to each selected peptide and the probability of interaction with Keap1 is represented here with a color scale, i.e., from lower (red) to higher (green). Characteristics of amino acids present in all peptides were determined by the Peptide Property Calculator database. Theoretical isoelectric point (pI) was calculated with ProtParam database [29]. Bold highlights the names of the peptides.