Table 4.

Antiparasitic activity of Origanum vulgare L. essential oil (OEO).

Parasite	Dose	Details about the Source of the Oil and Tested Concentration	Reference
Cryptosporidium parvum	At 60 μg/mL OEO reduced Cryptosporidium parvum infectivity to 55.6 ± 10.4%	OEO (origin: Turkey, steam extracted) was obtained from Oregano World, Hollywood. HPLC analysis of the OEO attested the presence of carvacrol (594.6 ± 10.0 μg/mL) Tested concentrations of OEO were: 0, 7, 15, 30, 60, 125, 250, 500 and 1000 μg/mL Infectivity was assessed via immunofluorescence detection using phase-contrast/fluorescent microscopy.	[54]
Trypanosoma cruzi	IC50 = 175 μg/mL—inhibited epimastigote growth IC50 = 115 μg/mL—induced trypomastigote lysis	Origanum vulgare L. was harvested from Lavras, Brazil. Qualitative and quantitative determination of the major phytocompounds were identified using GS-MS and GS-FID: 3-cyclohexen-1-ol (26.2%), γ-terpinene (16.0%), α-terpineol (12.3%) Concentrations of the OEO ranged from 25 to 250 μg/mL IC50 was determined after 24 h incubation by cell counting.	[55]
Echinococcus granulosus	OEO (10 μg/mL) diminished (p < 0.01) the viability of protoscoleces to 22.3 ± 1.2% after 60 days of incubation	Origanum vulgare L. was harvested from Buenos Aires, Argentina Qualitative and quantitative determination of the major phytocompounds were identified using GS-MS and GS-FID and confirmed the presence of: carvacrol (20.14%), thymol (19.71%), γ-terpinene (12.77%) In vitro viability was assessed by the methylene blue exclusion test.	[56]