Figure 3.

Morphological analysis of M12.C3.F6 cells treated with seasonal samples of CP (25 µg/mL) and H₂O₂ (0, 1 and 5 mM). Cellular antioxidant activity (CAA) was determined on morphologically and metabolically viable cells by forward scatter (FSC) and side scatter (SSC) parameters, according to treated cells, as well as by exclusion of PI staining. Quadrants Q3 and Q4 encompass viable and metabolically active cells under each tested condition (wherein at least 90–95% of cells were viable). Caffeic acid phenethyl ester (CAPE) was included as a positive control for CAA assays. Dot plots depicting morphological analysis are representative of at least three independent experiments.