Figure 3.

Phylogenetic analysis targeting the VP1/P2B junction of hepatitis A virus (HAV) strains detected in samples from all five wastewater treatment plants (WWTPs). Previously characterised HAV strains from clinical sources in Gauteng (●) and from other countries (●) are indicated. The names of type strains for each genotype and subgenotype are bolded and italicised. The neighbour-joining tree was constructed using the Kimura two-parameter model. Bootstrap values greater or equal to 70% are shown at the nodes. The nucleotide sequence alignment used to infer the analysis is provided as Supplementary file 2 (Fasta file).