Table 1.
Changes of normalized relative gene expression quantities in M. sativa stems upon cadmium (Cd) exposure. Normalized expression values are given relative to the control set at 1.00. Values are given as an average of 5 replicates. A t-test was performed to determine the significance (p ≤ 0.05). Green significantly up-regulated; red significantly down-regulated.
| Gene Annotation | Contig ID M. sativa | Rel. Norm. Gene Expression | p-Value |
|---|---|---|---|
| Polyamine synthesis | |||
| arginine decarboxylase | 46,745 | 1.123 | 0.548 |
| diamine oxidase | 14,769 | 0.800 | 0.042 |
| glutamate decarboxylase | 103,359 | 1.685 | 0.019 |
| SAM decarboxylase | 9152 | 1.141 | 0.459 |
| spermidine synthase | 5341 | 1.213 | 0.042 |
| spermine synthase | 92,526 | 1.341 | 0.032 |
| Flavone synthesis | |||
| chalcone isomerase | 60,809 | 1.809 | 0.019 |
| chalcone reductase | 59,796 | 2.753 | 0.019 |
| chalcone synthase | 99,463 | 1.247 | 0.371 |
| isoflavone synthase | 10,252 | 2.699 | 0.019 |
| Glutathione synthesis and redox cycle | |||
| homoglutathione synthetase | [63] | 1.355 | 0.01 |
| γ-glutamylcysteine synthetase | [63] | 0.959 | 0.361 |
| glutathione reductase 1 | [63] | 1.105 | 0.071 |
| glutathione reductase 2 | [63] | 1.043 | 0.681 |
| monodehydroascorbate reductase | [63] | 1.177 | 0.048 |
| glutathione synthetase 2 | 10,444 | 1.384 | 0.004 |