Figure 4.

Main metabolic pathways affected by 4 °C and 22 °C storage after 1, 3, 6, 8, 12, 24, and 48 h. Metabolites in bold are those detected in this work, by NMR; blue, variations at 4 °C; orange, variations at 22 °C; the symbol - represents a time point where no variation was noted compared to 0 h; ↑ and ↓ represent metabolite level increased and decreased compared to 0 h, respectively; so that for instance the indication - - - - - ↑↑ expresses no changes at 1, 3, 6, 8, and 12 h, and increases at 24 and 48 h. Three-letter code used for amino acids; GAA, guanidinoacetate; NAG, N-acetyl of glycoproteins; PEP, phosphonenolpyruvate.