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. 2020 Dec 17;25(24):5979. doi: 10.3390/molecules25245979

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of rHct-S3 on migration of HT-29 cells, MMPs and apoptotic proteins. (a,b) The HT-29 cells migration distance was measured the width of the wound and expressed as a percentage of each control for the mean of wound closure area. All experiments were repeated at least three times in each group (n = 18 for control and each compound, n—quantity of photos). The magnification of representative photos is ×10. The asterisks (*** p < 0.001) indicate a significant decrease in migration of cells treated with rHct-S3 compared with the non-treated cells (control) (c,d) rHct-S3 inhibited MMP-9, MMP-2 expression and regulated caspase-3, cleaved caspase-3, PARP, Bcl-2 and Bax in HT-29 cells, as determined by Western Blotting with specific antibodies.