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. 2020 Dec 20;9(12):1808. doi: 10.3390/plants9121808

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the interaction between PsBELL1-2, PsKNOX9 and PsBELL1-2, PsDELLA1 using different approaches. Yeast two-hybrid assays between PsBELL1-2 and PsKNOX9 (A), PsBELL1-2 and PsDELLA1 (C). Co-immunoprecipitation (Co-IP) between PsDELLA1-FLAG and PsBELL1-2 (B). For the yeast two-hybrid assays, serial dilutions optical density at 600 nm (OD600) = 0.1 and 0.05 were used. Interaction was tested on a selective medium lacking leucine, tryptophan, and histidine (SD-LTH) and medium additionally lacking adenine (SC-LTAH). Yeast growth on indicated mediums shows the protein interaction. As controls, a few pairs of vectors (pEXP32/Krev1 and pEXP22/RalGDS-wild type, pEXP22/RalGDS-m1, and pEXP22/RalGDS-m2) suggested by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA) were used for strong, weak, and not detectable interactions.