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. 2020 Dec 18;10(12):1692. doi: 10.3390/biom10121692

Figure 4.

Figure 4

NaHS co-treatment partially counteracts the dysregulation of the expression of local chaperones for the ER folding machinery. HepG2 cells were serum-starved for 8 h and treated with the vehicle or 100 nM of thapsigargin (TG), in the presence or absence of 100 µM NaHS for 16 h. Cells were harvested and the extracted proteins were processed for Western blotting analysis of the expression levels of calnexin (a), ER oxidoreductase 1α (ERO1-Lα) (b), and protein disulfide isomerase (PDI) (c). Each graph line represents mean ± SEM from three independent experiments. Data are expressed as a fold change of the control (vehicle-treated) conditions (CC). * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001 compared to CC; # p ≤ 0.05, ## p ≤ 0.01 compared to TG-treated cells.