Table 1.
List of primary antibodies used in Western blotting. (SM = skimmed milk; BSA: bovine serum albumin).
| Target | Blocking Buffer | Dilution | Isotype | Manufacturer | Catalogue Number |
|---|---|---|---|---|---|
| β-Actin | 5% w/v SM | 1:2000 | Mouse IgG | Cell Signaling | 3700 |
| Beclin-1 | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 3495 |
| Atg3 | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 3415 |
| Atg7 | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 8558 |
| ATF6 | 5% w/v SM | 1:1000 | Mouse IgG | Abcam | ab122897 |
| BiP | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 9956 |
| Calnexin | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 9956 |
| PDI | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 9956 |
| Ero1-Lα | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 9956 |
| Chop | 5% w/v SM | 1:1000 | Mouse IgG | Cell Signaling | 9956 |
| IRE1α | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 9956 |
| Phospho-IRE1 (Ser724) | 5% w/v SM | 1:1000 | Rabbit IgG | Abcam | ab48187 |
| PERK | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 3192 |
| Phospho-PERK (Thr980) | 5% w/v BSA | 1:1000 | Rabbit IgG | Thermo Fisher | 15033 |
| Phospho-eIF2α (Ser51) | 5% w/v BSA | 1:1000 | Rabbit IgG | Cell Signaling | 9721 |
| Bak | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 12105 |
| Bcl-xL | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 2764 |
| Cleaved PARP | 5% w/v SM | 1:500 | Rabbit IgG | Abcam | ab32064 |
| CBS | 5% w/v SM | 1:1000 | Rabbit IgG | Cell Signaling | 14782 |
| 3-MST | 5% w/v SM | 1:1000 | Rabbit IgG | Abcam | ab154514 |
| CSE | 5% w/v SM | 1:1000 | Rabbit IgG | Abcam | ab151769 |
| TST | 5% w/v SM | 1:1000 | Rabbit IgG | Abcam | ab166625 |
| ETHE1 | 5% w/v SM | 1:1000 | Rabbit IgG | Abcam | ab174302 |