Figure 5.

Characterization of CRISPR/Cas9^miR171f T₁ plants with homozygous mutations. (a) Mutation patterns were determined from CRISPR/Cas9^miR171f T₁ plants. T₁ generation obtained from three independent CRISPR/Cas9^miR171f transgenic plants was used to investigate the inheritance and segregation of mutations observed in the T₀ stage. (b) Prediction of the stem–loop structure of the mutated miR171f. The mature miR171f is shown in red. (c) Expression patterns of pri-miR171f and mature miR171f-5p in non-transgenic and CRISPR/Cas9^miR171f transgenic plants.