Table 1.

List of target genes and mutation patterns in corresponding transgenic plants.

Target Gene	gRNA	GC(%)	Tm	WT	Mono-Allelic	Biallelic		Total Plants	Mutation Rate (%)
						Homo	Hetero
miR399d	TCACCAAAACGGCCTGCCAAAGG	55	73.0	40			6	46	13.0%
miR418	AATTCCACCGTGGTCCCTGGAGG	60	70.5	26		6	10	42	38.1%
miR156d	AGAGTGAGCACACGGCGTGATGG	60	70.1	3		8	37	48	93.8%
miR399e	TGCCCAGCAATGCAACTTTGCGG	50	69.6	14	14	1	3	32	56.3%
miR399i	TGCTAGCCTTTCCCTGCCAAAGG	55	69.2	3	6	8	4	21	85.7%
miR169f	AAGAGCTGATTCGGTAGCCAAGG	50	64.2	7	3	10	29	49	85.7%
miR171f	TTGGCATGGTTCAATCAAACCGG	40	63.7	21	1	3	24	49	57.1%
OsNAC14	AAGAGCTCTGGTGCAAGAAGGGG	50	61.8	3	4	5	36	48	93.8%
miR156g	GAAGAGAGTGAGCACACAGCGGG	55	61.0	1	2	3	12	18	94.4%
miR399k	GGTTACCAGACTACTGCCAAAGG	50	59.7		3	7	20	30	100.0%
miR818b	ATCCAAAATCCCTTATATTATGG	25	52.5	17	2			19	10.5%
miR814a	ACTTCATAGTACAACGAATCTGG	35	51.3	19	3		1	23	17.4%
miR816	ATATTTTACTACAACGAATCTGG	25	48.2	32	1			33	3.0%
Total				186	39	51	182	458
				40.6%	8.5%	11.1%	39.7%