Figure 7.

Phase I and phase II enzyme activity after MgA treatment of hepatic spheroids exposed to L. infantum axenic promastigotes. Samples of 3D-culture supernatants were used to evaluate EROD (A), BROD (B), MROD (C), PROD (D), and UGT (E) enzyme activity at 1.5, 5, 24, and 72 h of MgA treatment. Untreated hepatocyte aggregates exposed to parasites for 72 h (LiPexp) also were evaluated. Results of 10 dogs performed in triplicate are represented by box plots, whiskers (minimum to maximum), and medium. The non-parametric Wilcoxon test was used for statistical comparisons, and * indicates statistically significant values (p < 0.05) when comparing untreated vs. treated amastigote-exposed spheroids.