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. 2020 Dec 17;10(12):357. doi: 10.3390/life10120357

Figure 2.

Figure 2

Heterozygous knockout of β-catenin leads to canonical Wnt signaling downregulation in the hearts of trained and untrained mice. (A) Western blot of total β-catenin, Lef-1, Axin-1, and adenomatous polyposis coli (APC) in left ventricle (LV) lysates from WT/WT and WT/CKO mice in sedentary conditions (Control) and after endurance training (Training). (B) Densitometry of total β-catenin normalized to total β-actin. (C) Densitometry of Lef-1 normalized to Gapdh. (D) Densitometry of Axin-1 normalized to β-actin. (E) Densitometry of APC normalized to β-actin. (F) qPCR analysis of β-catenin target gene expression. The data are expressed as the mean ± SD of arbitrary fold of change relative to control levels. n = 5/group. * p < 0.05, ** p < 0.01 (Kruskal–Wallis test followed by Dunn’s multiple-comparison post hoc test).