Figure 4.

Heterozygous deletion of the β-catenin leads to the inhibition of triacylglycerols (TAG) hydrolysis in the hearts of trained and untrained WT/CKO mice. (A) Thin layer chromatography of lipids extracted from LV from WT/WT and WT/CKO mice in sedentary conditions (Control) and after the endurance training (Training). (B) Densitometry of TAG. (C) Densitometry of diacylglycerols (DAG). (D) Densitometry of free fatty acids (FFA). (E) Western blot of ATGL, ABHD5, G₀S₂, pHSL at Ser⁵⁶³, pHSL at Ser⁵⁶⁵, total HSL, and in LV lysates from WT/WT and WT/CKO mice in sedentary conditions (Control) and after the endurance training (Training). (F) Densitometry of ATGL protein normalized to GAPDH. (G) Densitometry of G₀S₂ protein normalized to GAPDH. (H) Densitometry of ABHD5 protein normalized to Gapdh; (I) Densitometry of HSL protein normalized to GAPDH. (J) Densitometry of pHSL at Ser⁵⁶³ normalized to total HSL. (K) Densitometry of pHSL at Ser⁵⁶⁵ normalized to total HSL. (L) Densitometry of CD36 protein normalized to GAPDH. The data are expressed as the mean ± SD of arbitrary fold of change relative to control levels. n = 5/group. * p < 0.05, ** p < 0.01 (Kruskal–Wallis test followed by Dunn’s multiple-comparison post hoc test).