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. 2020 Dec 16;25(24):5964. doi: 10.3390/molecules25245964

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Neighboring group effect related enhancement in biocompatibility: (a) 4-TMR-COOH, 4-580CP-COOH, 4-610CP-COOH and 4-SiR-COOH dyes and conjugates. Their photo-physical properties are depicted in the color spectrum chart and the neighboring group effect is depicted; (b) Wide-field microscopy images of living primary fibroblasts, stained with 4/5/6-580CP-Hoechst (magenta) or 4/5/6-610CP-JAS (yellow); (c) Quantification of DNA probe fluorescence signal in the nuclei. Adapted with permission from [148]—Published by The Royal Society of Chemistry. Scale bars = 100 µm.

Neighboring group effect related enhancement in biocompatibility: (a) 4-TMR-COOH, 4-580CP-COOH, 4-610CP-COOH and 4-SiR-COOH dyes and conjugates. Their photo-physical properties are depicted in the color spectrum chart and the neighboring group effect is depicted; (b) Wide-field microscopy images of living primary fibroblasts, stained with 4/5/6-580CP-Hoechst (magenta) or 4/5/6-610CP-JAS (yellow); (c) Quantification of DNA probe fluorescence signal in the nuclei. Adapted with permission from [148]—Published by The Royal Society of Chemistry. Scale bars = 100 µm.