Table 1.

Summary of spectroscopic data of the newly synthesized dyes, including previously reported values for Tokyo Magenta [298] and Tokyo Green [98] for comparison.

Dyes		λabs [nm]	λem [nm]	Stokes Shift [nm]	ε [M−1cm−1]	Φ	Brightness (ε∙Φ) [M−1cm−1]	pKa
Rhodamine	NR675	675	695	20	73,200	0.24	17,568	<0.52
	NR669	669	689	20	77,200	0.30	23,160	<0.59
Rhodol	NR638	638	662	24	55,630	0.21	11,682	5.16
	NR632	632	655	23	63,970	0.26	16,632	5.20
Fluorscein	NR604	604	627	23	45,740	0.48	21.955	6.71
	NR600	600	619	19	67,570	0.68	45,948	6.75
	Tokyo Magenta	582	598	16	110,000	0.42	46,200	6.8
	Tokyo Green	491	510	19	85,000	0.85	72,250	6.2