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Photophysical properties of spontaneously blinking fluorophores with calculated and experimental values.
| λabs [nm] | λem [nm] | Exp. pKcycl | Calc. pKcycl | Exp. τcycl [ms] | Calc. τcycl [ms] | |
|---|---|---|---|---|---|---|
| HMRG | 500 | 523 | 8.1 | 7.9 | - | |
| 3MHMRG | 500 | 523 | 6.6 | 6.1 | 27.3 | 38.0 |
| M3MHMRG | 498 | 520 | 4.7 | <4 | 6.6 | 2.0 |
| HMCR550 | 559 | 582 | 5.4 | 5.7 | 40.9 | 78.6 |
| HMSiR600 | 595 | 615 | 4.4 | <4 | - | 5.3 |
| 5-COOH HMSiR600 | 595 | 615 | 4.7 | - | 11.5 | - |
| HMSiR | 650 | 671 | 5.8 | 6.2 | 375 | 165 |
