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. 2020 Dec 21;9(12):1810. doi: 10.3390/plants9121810

Figure 1.

Figure 1

The ability of Bacillus subtilis 10-4 (endophyte) to colonize internal tissues of surface-sterilized wheat plants. Two cultivars were tested: cv. Ekada70 (drought-tolerant)) (A) and cv. Salavat Yulaev (drought-sensitive) (B). i—the absence of B. subtilis bacteria growth around the surface-sterilized root and leaf segments in non-inoculated wheat seedlings (Control); ii—B. subtilis bacterial growth (* light green to green colonies) around the surface-sterilized leaf and root segments of wheat seedlings pre-inoculated with B. subtilis 10-4 (Endophyte); (C)—electrophoregram of PAAG: M—DNA marker; 1—DNA of origin B. subtilis 10-4, used for pre-sowing inoculation of seeds; 2 and 3—DNA of bacteria grown around wheat plant segments. Black arrows indicate B. subtilis growth (light green to green colonies) around the plant segments. Each variant was performed in three replicates, 10 seedlings per replicate.