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. 2020 Dec 21;21(24):9748. doi: 10.3390/ijms21249748

Figure 1.

Figure 1

Effect of Jakyakgamcho-Tang (JGTW) and 30% EtOH Jakyakgamcho-Tang (JGTE) on viability, nitric oxide (NO), prostaglandin (PG) E2 production, and inducible nitric oxide/cyclooxygenase-2 (iNOS/COX-2) expression of lipopolysaccharide (LPS)-induced RAW264.7 macrophages. RAW264.7 cells were pre-treated with JGTW or JGTE (50, 100, and 200 µg/mL) for 2 h, and then stimulated with LPS (0.5 µg/mL) for 24 h. (A) Cell viability was measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. (B) NO production was measured using a Griess reagent assay. L-NG-monomethyl arginine (L-NMMA) was used as a positive control. (C) PGE2 was measured using ELISA. The levels of (D) iNOS and (E) COX-2 were measured by real-time PCR. Values are expressed as means ± SD (n = 3). ### p < 0.001, #### p < 0.0001 vs. vehicle control cells. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. LPS-induced cells.