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Multiple gradient development program in the analysis of phenolic acids.
| Development Step | Mobile Phase Composition |
|---|---|
| 1, 2 | 3 mL solution A (heptane: dichlorometane, 7:3), 2 mL diisopropyl ether, 0.1 mL 85% formic acid, 1 mL distilled water |
| 3–7 | 4 mL solution A (heptane: dichlorometane, 7:3), 1 mL diisopropyl ether, 0.1 mL 85% formic acid |
